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Rudolph of Anhalt-Zerbst (Harzgerode, 28 October 1576 – Zerbst, 30 July 1621), was a German prince of the House of Ascania and ruler of the unified Principality of Anhalt. From 1603, he was ruler of the principality of Anhalt-Zerbst.

Rudolph was the fifth son of Joachim Ernest, Prince of Anhalt, but third-born son by his second wife Eleonore, daughter of Christoph, Duke of Württemberg.